IEDM

Session 26: Sensors, MEMS, and BioMEMS Technologies for Neural Activity Monitoring and DNA Analysis

Tuesday, December 5
Imperial Ballroom B
Co-Chairs: Duygu Kuzum, University of California, San Diego
Bernard Legrand, LAAS-CNRS, University of Toulouse

2:05 PM
26.1 Transparent Artifact-free Graphene Electrodes for Compact Closed-loop Optogenetics Systems, X. Liu, Y. Lu, E. Iseri, C. Ren, H. Liu, T. Komiyama, and D. Kuzum, University of California San Diego

In this work, we fabricated low impedance transparent graphene microelectrode arrays for artifact-free electrophysiological recordings. We demonstrated that transparent graphene electrodes eliminate light-induced artifacts during optical imaging and optogenetic stimulation. Finally, we designed a compact system incorporating both the graphene microelectrode array and fiber-coupled μLEDs for closed-loop optogenetic stimulation.

2:30 PM
26.2 High-yield passive Si photodiode array towards optical neural recording, D. Mao, J. Morley, Z. Zhang, M. Donnelly, and G. Xu, *University of Massachusetts Amherst

We demonstrate a high yield, passive Si photodiode array, aiming to establish a miniaturized optical recording device for in-vivo use. Our fabricated array features high yield (>90%), high sensitivity (down to 32 μW/cm2), high speed (1000 frame per second by scanning over up to 100 pixels), and sub-10uW power.

2:55 PM
26.3 Interactions of nanowires with cells and tissue (Invited), C. Prinz, Lund University

We report that arrays of vertical gallium phosphide nanowires are promising materials for biosensing in membranes and cells. Moreover, due to the exceptional control one can achieve over their geometrical and optic properties, we use nanowires to investigate the interactions of high aspect ratio nanoparticles with living cells and tissue.

3:15 PM Coffee Break

3:40 PM
26.4 Integration of FinFETs and 3D nanoprobes devices on a common bio- platform for monitoring electrical activity of single neurons., A. Casanova, M.-C. Blatche, F. Mathieu, L. Bettamin, H. Martin, D. Gonzalez-Dunia, L. Nicu, and G. Larrieu, Université de Toulouse

We propose to co-integrate high surface-to-volume ratio active (Fin-FETs) and passive devices (vertical nanowire-probes) on the same platform to monitor activity of single mammalian neurons. High signal-to-noise ratio were demonstrated (SNR=80 for intracellular configuration). The bio-platform was used to examine the effect of bio-chemical and electrical stimulations on neuronal activity.

4:05 PM
26.5 Direct characterization of circulating DNA in blood plasma using µLAS technology, R. Malbec, B. Chami, H. H. T. Ngo, A. Didelot*, F. Garlan*, S. Garrigou*, V. Taly*, L. Aeschbach***, E. Trofimenko***, V. Dion***, A. Boutonnet-Rodat**, F. Ginot** and A. Bancaud, Université de Toulouse, *Paris Descartes University, **Picometrics Technologies, ***University of Lausanne

Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients’ treatment. However, current cfDNA characterization is severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that the µLAS technology allows us to quantitatively characterize the size distribution of purified cfDNA in a few minutes, even when its concentration is as low as 1 pg/µL. Moreover, we show that DNA profiles can be directly measured in blood plasma with a minimal conditioning process to speed up considerably speed up the cfDNA analytical chain.

4:30 PM
26.6 Nanopores incorporating ITO electrodes for electrical gating of DNA at different folding states, X. Zhu, X. Wang, Z. Cao, Z. Ye, C. Gu, C. H. Jin, Y. Liu, Zhejiang University

Nanopore devices integrated with ITO gate electrodes are fabricated, producing pore diameters <10nm and lengths ~30nm. Translocation signals of λ-DNA reveal detailed signatures of various DNA folding states. The gate bias VG modulates the translocation events. As VG rises from - 0.5V to 0.5V, the count of folded-once events increases by ~5.5X relative to that of unfolded ones, indicating capability of electrically modulating the effective pore cross-section.