Session 10: Focus Session - Sensors, MEMS, BioMEMS Nanosensors for Disease Diagnostics

Monday, December 4
Imperial Ballroom B
Co-Chairs: Séverine Le Gac, University of Twente
Edwin Carlen, University of Tsukuba

1:35 PM
10.1 Moved to 40.6

2:00 PM
10.2 Development of High-frequency Bulk Acoustic Wave (BAW) Resonators as Biosensors and Bioactuators (Invited), X. Duan, S. Pan and W. Pang, Tianjin University

Using of bulk acoustic wave resonators for biosensing applications grows rapidly in recent years. In this review, we summarized the recent trend developing of these devices for biodetection from two aspects: 1) as biosensors to provide label- free measurement of biomarkers. 2) as bioacutuators to manipulate biomolecules and enhance biosensing performance.

2:25 PM
10.3 A Single Bacterium and Mammalian Cell Analysis by Ionic Current Measurements in a Microchannel (Invited), N. Kaji, M. Sano, S. Ito, H. Yasaki, T. Yasui, H. Yukawa, and Y. Baba, Nagoya University

A microfluidic device based on ionic current detection system for high-throughput and practical single bacteria and mammalian cell sizing was developed, and furthermore, discrimination of bacterial species and mammalian cell deformability was achieved. The highly precise sizing system based on blocking ionic current at narrow microchannel provided the information on antibiotic resistant strains of bacteria. Deformability changes associated with passage of adipose tissue- derived stem cells (ASCs) were also successfully detected by the device without any chemical or biological modification.

2:50 PM
10.4 Rapid Antibiotic Susceptibility Testing System: Life Saving bioMEMS Devices (Invited), H. Y. Jeong, E.-G. Kim*, S. Han*, G. Y. Lee, S. Han*, B. Jin*, T. Lim, H. C. Kim**, T. S. Kim**, D. Y. Kim* and S. Kwon, Seoul National University, *Quantamatrix Inc, **Seoul National University Hospital

For the prompt prescription of patients suffering from infectious diseases such as tuberculosis or bloodstream infection, a rapid antimicrobial susceptibility test (RAST) is highly necessary. This paper describe rapid antibiotic susceptibility test system composed of biochips and automated expert system, which can determine the antibiotic susceptibility of bacteria and mycobacteria derived from various parts of body. With RAST, antibiotic susceptibility was available in six hours, which was conventionally taking more than two days. Device design consideration, clinical verification, commercialization, and application of RAST system to infectious diseases are reviewed.

3:15 PM Coffee Break

3:40 PM
10.5 Microscale Profiling of Circulating Tumor Cells(Invited), R. Mohamadi and S. Kelley, University of Toronto

Microscale analysis has facilitated significant progress towards the development of approaches that enable the capture of rare circulating tumor cells (CTCs) from the blood of cancer patients. This is a critical capability for noninvasive tumor profiling. These advances have allowed the capture and enumeration of CTCs with unique sensitivity. However, it has become clear that simply counting tumor cells cannot provide the information that could help to make significant clinical decisions. CTCs are heterogeneous and they can change as they enter the bloodstream. Therefore, profiling of CTCs at single cell level is critical to unraveling the complex and dynamic properties of these potential cancer markers. In this paper we discuss new nanoparticle-enabled microscale technologies for CTC characterization, developed in our laboratory, which profiles CTCs based on their surface expression profile. Validation data presented here show that cancer cells with varying surface expression generate different binning profiles. We then applied the new technologies to reveal the dynamic phenotypes of CTCs in unprocessed blood from animal models. We will also discuss the application of these technologies in analyzing blood samples from cancer patients. While most technologies developed for analyzing CTCs are based on microscopic imaging we have developed and integrated new electrochemical sensors with our CTC capture strategies that enabled us to gather further molecular information on these rare cells.

4:05 PM
10.6 Encapsulated Organoids & Organ-on-a-chip platform for cancer modeling (Invited), N. Picollet-D’hahan, B. Laperrousaz, S. Porte,P. Obeid, A. Tollance, F. Kermarrec, C. Belda-Marin*, A. Romero-Millan, V. Haguet, D.K. Martin* and X. Gidrol, University Grenoble Alpes, INSERM, CEA-LETI, *TIMC-IMAG / CNRS UMR

This review highlights our major developments in the fields of organoids and organ-on-chip to address issues in fundamental and biomedical research by modeling development and cancer. We illustrate how contemporary miniaturized technologies (e.g. microfluidics, 3D scaffolding, 3D imaging) combined with RNAi-based organoids HTS (High-Throughput Screening), would help forming and analyzing consistent, efficient and reproducible organoids. Moreover, we illustrate the potential of engineered organ-on-chip devices for creating novel human organ and disease models, with a particular focus on “prostate-on-a chip” developments.

4:30 PM
10.7 Tissue Microenvironment and Cellular Imaging (Invited), S.S. Nasseri, S.M. Grist, S. Chen, Y.Y. Tam, P. Cullis, and K.C. Cheung, University of British Columbia

Small tissue constructs comprising several cell types within a three-dimensional environment can better mimic tissue, and may provide a better system to screen and validate drugs than current two-dimensional monolayer cultures. We developed a microfluidic flow-focusing method to rapidly and reproducibly create multicellular, 3-D spheroids that can better model several aspects of the tumour in vivo, including diffusion gradients of O2 and drugs. When evaluating effects of drugs on arrays of micro-tissues in high content screening, we will need to image deep within the tissues to assess parameters such as cell viability at the tissue cores or drug penetration into the tissue as a function of time. We have developed an on-chip method to rapidly clear arrays of 3-D cell cultures and micro-tissues, compatible with two-photon microscopy to track drug and nanomedicine penetration into the tissues.